In this study, field experiments were carried out on nine crops, including cucumber and rice, using four typical bacilli, including subtilis, amylolytic, lateral spore brevis and gelatinoid, and a compound preparation composed of the above two bacilli. The observed data revealed the following points: (1) the effect of various bactericides on the yield increase of different crops varied. The yield increase of Bacillus amylolytica ranged from 10.4% to 13.2%, while the preparation containing trichoderma showed a gain of 10.5% to 14.8%. (2) On the same crop, the yield increasing effects of different microbial inoculants were also significantly different, ranging from 7.5% to 15.8%, and all the differences were statistically significant, among which trichoderma was the most outstanding. (3) The compound bactericides showed superior efficiency compared with a single strain. Taking rice as an example, the single application of Bacillus subtilis and Bacillus gelatinus resulted in a yield increase of 10.3% and 10.7%, respectively, and the combined yield of the two could reach 16.1%, which was particularly obvious. (4) All tested bactericides significantly improved crop quality characteristics, and verified the potential advantages of multi-strain combination in bactericide formulation. This finding is consistent with the current trend of multi-strain combination research.

Xintong Wang. Application and Effect Evaluation of Microbial Fertilizer Strain. Nature Environmental Protection (2025), Vol. 6, Issue 1: 1-14. https://doi.org/10.38007/NEP.2025.060101.

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